■ 基本信息
別名: | Plasmid #48753 |
啟動(dòng)子: | CAG |
復(fù)制子: | pBR322 |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 轉(zhuǎn)座 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | Hyg
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pPB-CAG-empty-pgk-hph是一個(gè)哺乳細(xì)胞轉(zhuǎn)座表達(dá)質(zhì)粒。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
> MoCloF 5'-agcgaggaagcggaagagcg-3'
CCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCG
CAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGT
ATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGC
TCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCTTTAACCCTAGAAAGATAATCATATTGTG
ACGTACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATCGGTCTGTATATCGAGGTTTATT
TATTAATTTGAATAGATATTAAGTTTTATTATATTTACACTTACATACTAATAATAAATTCAACAAACAA
TTTATTTATGTTTATTTATTTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAACAAAACA
TAACTTCGTATAGCATACATTATACGAAGTTATCTCGACGCGGCCGCTCTAGCCTCGAGGCTAGAACTAG
TGGATCTCGAGCCCCAGCTGGTTCTTTCCGCCTCAGAAGCCATAGAGCCCACCGCATCCCCAGCATGCCT
GCTATTGTCTTCCCAATCCTCCCCCTTGCTGTCCTGCCCCACCCCACCCCCCAGAATAGAATGACACCTA
CTCAGACAATGCGATGCAATTTCCTCATTTTATTAGGAAAGGACAGTGGGAGTGGCACCTTCCAGGGTCA
AGGAAGGCACGGGGGAGGGGCAAACAACAGATGGCTGGCAACTAGAA
> F1ori-R 5'-AGGGAAGAAAGCGAAAGGAG-3'
GTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCgCGTCCCATT
CGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGC
GAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAA
ACGACGGCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCTTAACCCTAGAAAGATAGT
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTG
TGCATTTAGGACATCTCAGTCGCCGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCAC
TGATTTTGAACTATAACGACCGCGTGAGTCAAAATGACGCATGATTATCTTTTACGTGACTTTTAAGATT
TAACTCATACGATAATTATATTGTTATTTCATGTTCTACTTACGTGATAACTTATTATATATATATTTTC
TTGTTATAGATATCCAACTATGAAACATTATCATAAAGCAATAGTAAAGAGCCTTCAGTAAAGAGCAGGC
ATTTATCTAATCCCACCCCACCCCCACCCCCGTAGCTCCAATCCTTCCATTCAAAATGTAGGTACTCTGT
TCTCACCCTTCTTAACAAAGTATGACAGGAAAAACTTCCATGTGGACATCTTTATTGTTTAATAGATCAT
CAATTTCTCGACTATTCCTTTGCCCTCGGACGAGTGCTGGGGCGTCGGTTTCCACTATCGGCGAGTACTT
CTACACAGCCATCGGTCCA
