■ 基本信息
復(fù)制子: | PUC |
原核抗性: | Amp |
真核抗性: | 綠色熒光蛋白GFPuv |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: | ORF
|
片段物種: |
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原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: | 綠色
|
■ 質(zhì)粒簡(jiǎn)介
pGFPuv是一個(gè)大腸桿菌綠色熒光表達(dá)質(zhì)粒。pGFPuv carries the“cycle 3”variant of GFP described by Crameri et al. (1). This gene was cloned between the two MCSs of the pUC19 derivative pPD16.43 (2). The GFPuv gene canbe easily excised from pGFPuv. Alternatively, the GFPuv coding sequence can be amplified by PCR. The GFPuv gene was inserted in frame with the lacZ initiation codon from pUC19 so that a β-galactosidase-GFPuv fusionprotein is expressed from the lac promoter in E. coli . Note, however, that if you excise the GFPuv coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode the native (i.e.,non-fusion) GFPuv protein. The pUC backbone of pGFPuv provides a high copy number origin of replication and ampicillin resistance gene for propagation in E. coli.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1842 pGFPuv大腸熒光質(zhì)粒.txt
