■ 基本信息
別名: | Plasmid #13573;金黃葡萄球拷貝整合 |
復(fù)制子: | Ec |
質(zhì)粒大小: | 5505bp |
原核抗性: | Amp |
真核抗性: | Chl |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 金黃色葡萄球菌 |
5'測(cè)序引物: | 根據(jù)全序列設(shè)計(jì) |
3'測(cè)序引物: | 根據(jù)全序列設(shè)計(jì) |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 金黃色葡萄球菌 |
質(zhì)粒用途: | 蛋白表達(dá)
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片段類型: | ORF
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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篩選標(biāo)記: | Chl
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■ 質(zhì)粒簡(jiǎn)介
pLI50質(zhì)粒是一個(gè)金黃色葡萄球菌單拷貝整合載體，在大腸桿菌里氨芐抗性，在金黃色葡萄球菌里是氯霉素抗性。參考文獻(xiàn)：Construction of single-copy integration vectors for Staphylococcus aureus.
Single-copy integration vectors suitable for cloning in Staphylococcus aureus have been constructed. Their construction was based on the site-specific recombination system of staphylococcal phage, L54a. The vectors are capable of autonomous replication in Escherichia coli, but they are not endowed with a replication function in S. aureus. As a consequence, establishment of these vectors in S. aureus can only be achieved by the integration system of the phage. Once integrated into the chromosome, the vectors, or their derivatives, were stably inherited even without selective pressure. Because such a vector exists in an integrated form in S. aureus, the gene dosage of the DNA cloned in the vector matches that of the chromosome.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1134pLI50金黃色葡萄球菌單拷貝整合質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
