■ 基本信息
啟動(dòng)子: | T7Phi3.8 |
復(fù)制子: | p15 |
質(zhì)粒分類(lèi): | 有毒蛋白表達(dá)配套質(zhì)粒 |
質(zhì)粒大小: | 4886bp |
質(zhì)粒標(biāo)簽: | T7 lysozyme |
原核抗性: | Chl |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 大腸桿菌 |
培養(yǎng)條件: | 參考相關(guān)文獻(xiàn) |
5'測(cè)序引物: | 根據(jù)序列設(shè)計(jì) |
3'測(cè)序引物:
| 根據(jù)序列設(shè)計(jì) |
備注: | 低拷貝 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 廣宿主 |
質(zhì)粒用途: |
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片段類(lèi)型: |
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片段物種: |
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原核抗性: | Chl |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1, 2). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.Unique sites are shown on the circle map.
1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
2. Chang, A.C.Y. and Cohen, S.N. (1978) J. Bacteriol. 134, 1141.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載
ZK1125pLysS質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
