■ 基本信息
啟動子: | PH |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 昆蟲細(xì)胞載體；桿狀病毒組成型表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 5468bp |
質(zhì)粒標(biāo)簽: | N-3C，N-GST |
原核抗性: | Amp |
真核抗性: | 慶大霉素Gentamicin |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 昆蟲細(xì)胞 |
誘導(dǎo)方式: | 組成型表達(dá)，無需誘導(dǎo) |
5'測序引物: | pFastbac-F:TATTCCGGATTATTCATACC |
3'測序引物:
| pFastBac-R:ACAAATGTGGTATGGCTGA |
備注: | 可表達(dá)GST融合蛋白 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡介
The Bac-to-Bac? Baculovirus Expression System provides a rapid and efficient method to generate recombinant baculoviruses (Ciccarone et al., 1997). This method was developed by researchers at Monsanto, and is based on site-specific transposition of an expression cassette into a baculovirus shuttle vector (bacmid) propagated in E. coli (Luckow et al., 1993). The major components of the Bac-to-Bac? Baculovirus Expression System include:
? A choice of pFastBac? donor plasmids that allow generation of an expression construct containing the gene of interest where expression of the gene of interest is controlled by a baculovirus-specific promoter.
? An E. coli host strain, DH10Bac?, that contains a baculovirus shuttle vector (bacmid) and a helper plasmid, and allows generation of a recombinant bacmid following transposition of the pFastBac? expression construct.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1041pFastBac-GST-C昆蟲胞內(nèi)質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
