■ 基本信息
啟動(dòng)子: | LEU2，T7,T3 |
復(fù)制子: | pUC |
質(zhì)粒分類: | 酵母系列，釀酒酵母表達(dá)載體 |
質(zhì)粒大小: | 6018bp |
原核抗性: | Amp |
真核抗性: | LEU2 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母細(xì)胞 |
誘導(dǎo)方式: | 半乳糖誘導(dǎo) |
5'測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | LEU2
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
This is one of a series of pBluescriptbased YCtype(centromeric) yeast shuttle vectors (ATCC 7714277145) differing in the yeast selectable marker gene. This vector carries the LEU2 selectable marker. It also encodes the beta galactosidase alpha peptide for bluewhite color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. It is useful in plasmid shuffling experiments. It was constructed by inserting a 2.235 kb fragment containing the LEU2 gene into the NdeI site of the pRSS56 vector and also inserting a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) into the AatII site of the same vector. All ends were blunted. The pRSS56 vector was constructed by ligating a PvuI fragment (bp 4982412) of pBluescript KS+ and the fragment from the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: LEU2 f1 ori (NaeI) T7 promoter lacZ/MCS T3 promoter pMB1 ori bla CEN6 ARSH4.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK957pRS315釀酒酵母質(zhì)粒.txt
