■ 基本信息
啟動子: | AOX1 |
復(fù)制子: | pUC |
終止子: | CYC1 |
質(zhì)粒分類: | 酵母系列，畢赤酵母表達載體 |
質(zhì)粒大小: | 7898bp |
原核抗性: | Amp |
真核抗性: | ADE2 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 酵母細胞 |
5'測序引物: | AOX5:GACTGGTTCCAATTGACAAGC |
3'測序引物: | AOX3:GGCAAATGGCATTCTGACAT |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 蛋白表達 |
片段類型: |
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
The PichiaPink? Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink? Yeast Expression System. The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink? strains. The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted. You can order the PichiaPink? Secreted Vector Kit to refill your PichiaPink? Yeast Expression System Kit.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK934pPinkα-HC畢赤酵母質(zhì)粒.txt
