■基本信息
啟動(dòng)子: | 無(wú)，需要克隆進(jìn)啟動(dòng)子才能表達(dá) |
復(fù)制子: | pVS1 |
質(zhì)粒大小: | 8958bp |
原核抗性: | Kan |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 植物細(xì)胞 |
5'測(cè)序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測(cè)序引物: | M13R |
備注: | 農(nóng)桿菌介導(dǎo) |
■質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | Hyg
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熒光標(biāo)記: |
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■質(zhì)粒簡(jiǎn)介
The pCambia vector backbone is derived from the pPZP vectors. The pCambia1300 vector offers:1.high copy number in E.coli for high DNA yields2.pVS1 replicon for high stability in Agrobacterium 3.small siz 4.restriction sites for modular plasmid modifications and small but adequate polylinkers for introducing DNA of interest 5.bacterial selection with kanamycin 6. plant selection with hygromycin B.
■質(zhì)粒圖譜
■質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK857pCAMBIA1300植物表達(dá)質(zhì)粒.txt
