■ 基本信息
啟動子: | CMV |
復制子: | pUC |
質(zhì)粒大小: | 7567bp |
原核抗性: | Amp |
真核抗性: | EGFP |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞,慢病毒 |
質(zhì)粒用途: | 基因沉默 |
片段類型: | shRNA
|
片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: | 綠色
|
■ 質(zhì)粒簡介
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
（pSico shRNA cloning protocol點擊下載）
pSicoshRNAcloningprotocol.pdf
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK826pSicoR慢病毒干擾質(zhì)粒.txt
