■ 基本信息
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒大小: | 10186bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 293T等哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 蛋白表達 |
片段類型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: | Hyg
|
熒光標記: |
|
■ 質粒簡介
pCEP4 is an episomal mammalian expression vector that uses thecytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.
pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK712pCEP4哺乳表達質粒.txt
