■ 基本信息
啟動子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞，熒光蛋白報告載體 |
質(zhì)粒大小: | 4793bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時表達(dá) |
5'測序引物:
| 根據(jù)序列設(shè)計引物
|
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 定位 |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | G418
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pEYFP-Mem encodes a fusion protein consisting of the N-terminal 20 amino acids of neuromodulin,also called GAP-43, and a yellow-green fluorescent variant of the enhanced green fluorescent protein (EGFP). The neuromodulin fragment contains a signal for posttranslational palmitoylation of cysteines 3 and 4 that targets EYFP to membranes. The EYFP gene contains four amino acid substitutions previously published as GFP-10C. The fluorescence excitation maximum of EYFP is 513 nm, and the emission maximum is 527 nm (in the yellow-green region).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK530pEYFP-Mem細(xì)胞膜定位質(zhì)粒.txt
