■ 基本信息
別名: | 有背景;Plasmid #22010 |
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | hGH poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞，雙分子熒光互補(bǔ)載體 |
質(zhì)粒大小: | 5216bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測(cè)序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 信號(hào)報(bào)告 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡介
BiFC-VN173 by pFlag-CMV Xba I and BamHI carrier in the site of insertion of the VN173 gene, the plasmid is high copy plasmid, VN173 gene derived from Vitoria victoria. Visualization of Aequorea Aequorea ternary complexes in living cells by using a BiFC-based FRET analysis. Nature Protocols, 3:1693-1702 (2008).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK517pBiFC-VN173雙分熒光質(zhì)粒.txt
