■ 基本信息
別名: | pGL4.73 |
啟動(dòng)子: | SV40 |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞，信號(hào)通路報(bào)告載體 |
質(zhì)粒大小: | 3921bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃ |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物:
| RVP3:CTAGCAAAATAGGCTGTCCC |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 信號(hào)報(bào)告 |
片段類型: | Promoter
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: | Rluc
|
■ 質(zhì)粒簡(jiǎn)介
The pGL4.73[hRluc/SV40] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.The pGL4.73[hRluc/SV40] Vector contains the hRluc reporter gene and an SV40 early enhancer/promoter and can be used as an expression control or a co-reporter vector.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK483pGL4.73[hRluc SV40]哺乳報(bào)告質(zhì)粒.txt
