■ 基本信息
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 哺乳細胞，信號通路報告載體 |
質粒大小: | 4283bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | 根據(jù)序列設計引物 |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 信號報告 |
片段類型: | Promoter |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
|
熒光標記: | Fluc
|
■ 質粒簡介
The pGL4.23[luc2/minP] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4.23[luc2/minP] Vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and a luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow for selection in E. coli.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK477pGL4.23[luc2-minP]哺乳報告質粒.txt
