■ 基本信息
別名: | pCDNA3.1+ |
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | BGH poly(A) signal |
質(zhì)粒分類: | 哺乳系列質(zhì)粒；哺乳表達(dá)質(zhì)粒；pCDNA系列質(zhì)粒 |
質(zhì)粒大小: | 5428bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，5%CO2 |
表達(dá)宿主: | 293T等哺乳細(xì)胞 |
誘導(dǎo)方式: | 無需誘導(dǎo) |
5'測序引物: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
3'測序引物: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pCDNA3.1(+)是來自pCDNA3的5.4kb載體，設(shè)計(jì)用于哺乳動(dòng)物宿主中的高水平穩(wěn)定和瞬時(shí)表達(dá)。大多數(shù)哺乳動(dòng)物細(xì)胞可以進(jìn)行高水平的穩(wěn)定和非復(fù)制性瞬時(shí)表達(dá)。人類巨細(xì)胞病毒立即早期（CMV）啟動(dòng)子，用于在廣泛哺乳動(dòng)物細(xì)胞中的高水平表達(dá)。前向（+）和反向（-）方向上的多個(gè)克隆位點(diǎn)，以促進(jìn)克隆用于選擇穩(wěn)定細(xì)胞系的新霉素抗性基因。在潛伏感染SV40或表達(dá)SV40大T抗原（例如COS-1，COS-7）的細(xì)胞系中的異常復(fù)制。質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
? Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
? Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning
? Neomycin resistance gene for selection of stable cell lines
? Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.
This pcDNA3.1(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin? selectable marker and a forward-orientation multiple cloning site.
pCDNA3.1(+)的參考文獻(xiàn)：Clinical significance of bromodomain containing protein 7 and its association with tumor progression in prostate cancer.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK347pCDNA3.1(+)哺乳表達(dá)質(zhì)粒.txt
