■ 基本信息
啟動子: | T7 promoter |
復(fù)制子: | Ori，F(xiàn)1 ori |
終止子: | rrnB T1 terminator |
質(zhì)粒分類: | 大腸桿菌質(zhì)粒；大腸其它質(zhì)粒；其它大腸質(zhì)粒 |
質(zhì)粒大小: | 6495bp |
質(zhì)粒標(biāo)簽: | SNAP-tag |
原核抗性: | Amp |
| 克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá)
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片段類型: | ORF
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pSNAP-Tag(T7)2是一個大腸桿菌表達(dá)質(zhì)粒，T7啟動子驅(qū)動SNAP標(biāo)簽和目的基因融合表達(dá)。pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag? protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag(T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the Nor C-terminus of the SNAP-tag. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6 - alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAPtag substrates.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK342pSNAP-Tag(T7)2大腸表達(dá)質(zhì)粒.txt
